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plasmid pcdna3 1 myc snap gp135  (Addgene inc)


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    Structured Review

    Addgene inc plasmid pcdna3 1 myc snap gp135
    Plasmid Pcdna3 1 Myc Snap Gp135, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pcdna3+1+myc+snap+gp135/pAG202+SFRS12+3'UTR+(Plasmid+%2312013)/pm41059339-53-14-17
    Average 93 stars, based on 6 article reviews
    plasmid pcdna3 1 myc snap gp135 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: Posttranslational microtubule modification alters podocalyxin-trafficking in epithelial cells.
    Article Snippet: .. To generate MDCK and MDCKΔTTL cells expressing PDX fused with the SNAP-tag (PDX-SNAP), the plasmid pCDNA3.1 (+)-myc-SNAP-GP135 (Addgene, Watertown, United States, (Castillon et al., 2013)) was transfected using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, United States) according to the manufacturer’s instructions. .. Stable cell clones expressing PDX-SNAP were selected as described previously (Zink et al., 2012) and are listed in Table 1. cDNA isolation, RT-qPCR analysis and ammonium chloride assay MDCK and MDCKΔTTL cells were cultured for 6 days, lysed and RNA was purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany).

    Article Title: Posttranslational microtubule modification alters podocalyxin-trafficking in epithelial cells
    Article Snippet: .. To generate MDCK and MDCK ΔTTL cells expressing PDX fused with the SNAP-tag (PDX-SNAP), the plasmid pCDNA3.1 (+)-myc-SNAP-GP135 (Addgene, Watertown, United States, ( )) was transfected using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, United States) according to the manufacturer’s instructions. ..

    Plasmid Preparation:

    Article Title: Posttranslational microtubule modification alters podocalyxin-trafficking in epithelial cells.
    Article Snippet: .. To generate MDCK and MDCKΔTTL cells expressing PDX fused with the SNAP-tag (PDX-SNAP), the plasmid pCDNA3.1 (+)-myc-SNAP-GP135 (Addgene, Watertown, United States, (Castillon et al., 2013)) was transfected using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, United States) according to the manufacturer’s instructions. .. Stable cell clones expressing PDX-SNAP were selected as described previously (Zink et al., 2012) and are listed in Table 1. cDNA isolation, RT-qPCR analysis and ammonium chloride assay MDCK and MDCKΔTTL cells were cultured for 6 days, lysed and RNA was purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany).

    Article Title: Posttranslational microtubule modification alters podocalyxin-trafficking in epithelial cells
    Article Snippet: .. To generate MDCK and MDCK ΔTTL cells expressing PDX fused with the SNAP-tag (PDX-SNAP), the plasmid pCDNA3.1 (+)-myc-SNAP-GP135 (Addgene, Watertown, United States, ( )) was transfected using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, United States) according to the manufacturer’s instructions. ..

    Transfection:

    Article Title: Posttranslational microtubule modification alters podocalyxin-trafficking in epithelial cells.
    Article Snippet: .. To generate MDCK and MDCKΔTTL cells expressing PDX fused with the SNAP-tag (PDX-SNAP), the plasmid pCDNA3.1 (+)-myc-SNAP-GP135 (Addgene, Watertown, United States, (Castillon et al., 2013)) was transfected using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, United States) according to the manufacturer’s instructions. .. Stable cell clones expressing PDX-SNAP were selected as described previously (Zink et al., 2012) and are listed in Table 1. cDNA isolation, RT-qPCR analysis and ammonium chloride assay MDCK and MDCKΔTTL cells were cultured for 6 days, lysed and RNA was purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany).

    Article Title: Posttranslational microtubule modification alters podocalyxin-trafficking in epithelial cells
    Article Snippet: .. To generate MDCK and MDCK ΔTTL cells expressing PDX fused with the SNAP-tag (PDX-SNAP), the plasmid pCDNA3.1 (+)-myc-SNAP-GP135 (Addgene, Watertown, United States, ( )) was transfected using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, United States) according to the manufacturer’s instructions. ..



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